MicrofluidicSP1.2Platform詳細(xì)配置如下：▲MicroscopeAxiovert40CFL:Contrastoption:Brightfield,Phase,Varel,Fluorescence(TRITC,FITC,etc).Shutters:AutomatedBrightfieldandFluorescence.Objectives:10xLDA-Plan,20xLDA-Plan,32xCP-Achromat,40xLDA-Plan.Eyepieces:E-PL10x/20Br.,10x/20Br.foc.Stage:Vena8biochipcompatiblestageholder,Eppendorftubesholder.▲Mirus1.0NanopumpSpecifications:ShearStressRangeof0.05 20dyne/cm2,VolumetricFlowRates100nL 20 L/minute(100 Lsyringe),SampleVolumeIncrementsFreelyadjustableLinearVelocityRangeof10 m/sto10cm/s;FlowDirectionReversible;SampleVolumeAspirationAccuracy 1%;ShearStressAccuracy 0.5%;SampleVolumeAspirationPrecision 1%.Multichannel:16-WaymanifoldallowingindividualaddressingmicrochannelsofVena8biochip.▲MotorizedstagewithZ-focusmodule.Specifications:MarzhauserIMseriesstage,Travel120mmx100mm,Repeatability 1um,Precision 3 m,MaximumTravelspeed180mm/s.▲MicroscopeCageincubator:Capabilities:Temperaturecontrol 0.10C,CO2andhumiditymodule.Options:Temperaturemonitoringsoftwaremodule,blackpanelsforuseinfluorescentmicroscopy.▲DeltapixDP450USB2.0cameraSpecifications:2MegaPixels(1,616x1,216);ColorCDD;1/1.8 format;Pixel4.4 mx4.4 m.Resolution:12FPS@1,616x1,216pixels;14FPS@1,280x1,024pixels;18FPS@1,024x768pixels;22FPS@800x600pixels;26FPS@640x480pixels.DynamicRange 66dB.▲DellOptiplex745PCcomputerConfiguration:IntelCore2processor;1.86GHz;2GBRAM;ATIRadeonX1300PRO;ObjectiveImagingstagecontrolhardware;19 LCDmonitor.WindowsXPProfessionalSP2;DeltaPixViewerSoftware;VenaFluxAssayandDucoCellsoftwarepreinstalled.▲VenaFluxAssaySoftware.Capabilities:Executionofpresetprotocols,simultaneouscontrolofpumping,scanningandimageacquisitions,sampletransferandprotocolflow.Intuitivestepwiseprotocolinterfacewithstepshierarchyandstatuscontrol.Office2007GUIinterface,presetanduser-designedprotocols.▲DucoCellsoftwareSpecifications:AutomaticCountingandDetection;Morphologicalparametersanalyzedautomatically(Area,diameter,form-factor,ellipticity,eccentricity,orientation,perimeter,asymmetry,cellcentroidcoordinates,ellipticalaxis);Cellsortingandfiltering;Abilitytoanalyzesub-populationsbasedoninclusion/exclusionofmorphologicalparameters.DatamaybeexportedtoExcelspreadsheetincorporatingautomaticgraphgeneration(e.g.%celladhesionvs.shearstress).FileFormatsanalyzed*.jpg,*.jpeg,*.tif,*.tiff,*.bmp,*.gif,*.png.▲Vena8biochipspackof10Specifications:MinimumSampleVolume3 L;MaximumSampleVolume100 L;ShearStressRangeof0.05 20dyne/cm2,stepsof0.05dyne/cm2.Numberofchannelsperbiochipis8;Volumeofeachchannelis0.8 L.Deadvolumeatinputportis0.1 L.Vena8biochipdimensionsare400 m(W)x100 m(D)x20mm(L).