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Cyanine3 NHS ester
? ? Cyanine3 NHS ester是標(biāo)記生物分子的氨基基團(tuán)的活性染料，是Cy3? NHS ester的類似物。該熒光染料是可溶性蛋白、多肽和寡核苷酸/DNA的理想標(biāo)記物。對于痕量蛋白質(zhì)，可以考慮用水溶的不需要共溶劑的sulfo-Cyanine3 NHS ester。Cyanine3 NHS ester可以替代Cy3?, Alexa Fluor 546、DyLight 549。
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一般特性
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外觀: |
red powder |
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質(zhì)譜分析M+增量: |
474.2 |
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分子量: |
590.15 |
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分子式: |
C34H40ClN3O4 |
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溶解性: |
soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
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質(zhì)量控制: |
NMR 1H and HPLC-MS (95%) |
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儲存條件: |
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
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光譜特性
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?最大激發(fā)波長, nm: |
555 |
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在最大激發(fā)波長下的消光系數(shù), Lmol-1cm-1: |
150000 |
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最大發(fā)生波長, nm: |
570 |
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熒光量子產(chǎn)率: |
0.31 |
Sulfo-Cyanine7 maleimide
?A reactive dye with a maleimide group for the labeling of thiol groups of proteins and other biomolecules, an analog of Cy7? maleimide.Sulfo-Cyanine7 is a near infrared fluorophore which is hydrophilic and contains a rigidizing ring in its heptamethine chain that improves its photophysical properties. A great dye for various applications, including NIR imaging.
General properties
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Appearance: |
dark green powder |
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Mass spec M+ increment: |
868.2 |
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Molecular weight: |
869.10 |
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Molecular formula: |
C43H49KN4O9S2 |
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IUPAC name: |
1-[2-(6-{2-[(E)-2-[(E)-3-{(E)-2-[1-Methyl-3,3-dimethyl-5-(oxysulfonyl)-2-indolinylidene]ethylidene}-1-cyclohexen-1-yl]ethenyl]-3,3-dimethyl-5-(oxysulfonyl)-3H-indol-1-yl}hexanoylamino)ethyl]-1H-pyrrole-2,5-dione, potassium salt |
|
Solubility: |
Good in water, DMF, DMSO. Low in non-polar organic solvents. |
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Quality control: |
NMR?1H, HPLC-MS (95%) |
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Storage conditions: |
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
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Spectral properties
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Excitation maximum, nm: |
750 |
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ε, L?mol?1?cm?1: |
240600 |
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Emission maximum, nm: |
773 |
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CF260: |
0.04 |
|
CF280: |
0.04 |
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其他活性熒光染料產(chǎn)品目錄? ? ? ?
N-羥基琥珀酰亞胺脂Dye NHS esters
§??Cyanine3 NHS ester
§??Cyanine3.5 NHS ester
§??Cyanine5 NHS ester
§??Cyanine5.5 NHS ester
§??Cyanine7 NHS ester
§??Cyanine7.5 NHS ester
§??Sulfo-Cyanine3 NHS ester
§??Sulfo-Cyanine5 NHS ester
§??Sulfo-Cyanine7 NHS ester
疊氮化物?Dye azides
§??Cyanine3 azide
§??Cyanine3.5 azide
§??Cyanine5 azide
§??Cyanine5.5 azide
§??Cyanine7 azide
§??Cyanine7.5 azide
§??Sulfo-Cyanine3 azide
§??Sulfo-Cyanine5 azide
§??Sulfo-Cyanine7 azide
馬來酰亞胺?Dye maleimides
§??Cyanine3 maleimide
§??Cyanine5 maleimide
§??Cyanine5.5 maleimide
§??Cyanine7 maleimide
§??Cyanine7.5 maleimide
§??Sulfo-Cyanine3 maleimide
§??Sulfo-Cyanine5 maleimide
§??Sulfo-Cyanine7 maleimide
炔烴?Dye alkynes
§??Cyanine3 alkyne
§??Cyanine5 alkyne
§??Cyanine5.5 alkyne
§??Cyanine7 alkyne
§??Cyanine7.5 alkyne
§??Sulfo-Cyanine3 alkyne
§??Sulfo-Cyanine5 alkyne
§??Sulfo-Cyanine7 alkyne
胺基Primary amine
§??Cyanine3 amine
§??Cyanine5 amine
§??Cyanine5.5 amine
§??Cyanine7 amine
§??Cyanine7.5 amine
§??Sulfo-Cyanine3 amine
§??Sulfo-Cyanine5 amine
§??Sulfo-Cyanine7 amine
羧酸?Carboxylic acids
§??Cyanine3 carboxylic acid
§??Cyanine3.5 carboxylic acid
§??Cyanine5 carboxylic acid
§??Cyanine5.5 carboxylic acid
§??Cyanine7 carboxylic acid
§??Cyanine7.5 carboxylic acid
§??Sulfo-Cyanine3 carboxylic acid
§??Sulfo-Cyanine5 carboxylic acid
§??Sulfo-Cyanine7 carboxylic acid
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